Starting off with optimizing a PCR can be a daunting task. Optimization outcomes vary widely with reaction temperatures, reagent consistency and sometimes even pipetting technique. The following guide will help you to optimize your PCR results in no time. Image Credits: Lab Manager 1. Primer Selection Primer selection is the first step towards a successful PCR is primer selection. There are two ways you can go with this. The first is to select primers that have been already reported in previous publications. It is a shortcut but care must be taken to select primers that have been reported in a large number of current publications. The second way that you can go around is with designing a primer of your own. Online programs such as the primer designing tool by NCBI can help you create new primers and also ensure the integrity of previously reported primers. 2. Order Multiple Primers When ordering custom made primers for a particular gene, you c...
They say a pipette is a scientist's most powerful weapon.